毕赤酵母
人血清白蛋白
重组DNA
磁性纳米粒子
化学
白蛋白
纳米颗粒
色谱法
材料科学
生物化学
纳米技术
基因
作者
Seyedeh-Masoumeh Taghizadeh,Alireza Ebrahiminezhad,Mohammad Bagher Ghoshoon,Ali Dehshahri,Aydin Berenjian,Younes Ghasemi
出处
期刊:Nanomaterials
[Multidisciplinary Digital Publishing Institute]
日期:2020-01-06
卷期号:10 (1): 111-111
被引量:20
摘要
Magnetic immobilization as a novel technique was used to immobilize recombinant Pichia pastoris (GS115 Albumin) cells to produce human serum albumin (HSA). In this regard, magnetic nanoparticles (MNPs) coated with amino propyl triethoxy silane (APTES) were synthesized. P. pastoris cells were decorated with MNPs via nonspecific interactions. Decorated cells were magneto-responsible and easily harvested by applying an external magnetic field. The efficiency of magnetic immobilization (Ei) for cell removal was in direct relation with the MNP concentration and time of exposure to the magnetic field. By increasing the nanoparticles concentration, cells were harvested in a shorter period. Complete cell removal (Ei ≈ 100) was achieved in ≥0.5 mg/mL of MNPs in just 30 s. HSA is produced in an extremely high cell density (OD ~20) and it is the first time that magnetic immobilization was successfully employed for harvesting such a thick cell suspension. After 5 days of induction the cells, which were immobilized with 0.25 to 1 mg/mL of nanoparticles, showed an increased potency for recombinant HSA production. The largest increase in HSA production (38.1%) was achieved in the cells that were immobilized with 0.5 mg/mL of nanoparticles. These results can be considered as a novel approach for further developments in the P. pastoris-based system.
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