The expression of glucagon-like peptide 1 receptor in human hepatic stellate cell

Objective To confirm the expression of glucagon-like peptide 1 (GLP-1) receptor in human hepatic stellate cell (HSC).Methods Human HSC was cultured in vitro and morphologically identified.The mRNA of HSC was extracted and reversely transcribed to obtain cDNA.The mRNA of GLP-1 receptor was determined by RT-PCR.The GLP-1 receptor protein was determined by western blotting.Results The activating HSC were flat,with large cell body and well-developed stress fibers and without fat droplets in the cytoplasm under the microscope.The length of GLP-1 receptor cDNA fragment was 296 bp,which was 100％ consistent with the human GLP-1 receptor eDNA sequence (NM-002062.3) published in Gen Bank.The results of western blotting showed that there was GLP-1 receptor protein expressed in human HSC.Conclusions The GLP-1 receptor express in human HSC. Key words: Hpatic stellate cell;  GLP-1 receptor;  Western bloting;  RT-PCR;  Anti-hepatic fibrosis