Protein Chromatography

Preface DOWNSTREAM PROCESSING OF BIOTECHNOLOGY PRODUCTS Introduction Bioproducts and their Contaminants Bioprocesses Role of Chromatography in Downstream Processing References INTRODUCTION TO PROTEIN CHROMATOGRAPHY Introduction Basic Principles and Definitions Modes of Operation Performance Factors Separation Performance Metrics CHROMATOGRAPHY MEDIA Introduction Interaction Types and Chemistry Buffers and Mobile Phases Physical Structure and Properties LABORATORY AND PROCESS COLUMNS AND EQUIPMENT Introduction Laboratory-scale Systems Process Columns and Equipment ADSORPTION EQUILIBRIA Introduction Single Component Systems Multi-component Systems References ADSORPTION KINETICS Introduction Rate Mechanisms Batch Adsorption Kinetics DYNAMICS OF CHROMATOGRAPHY COLUMNS Introduction Conservation Equations Local Equilibrium Dynamics Multi-component Systems Displacement Development EFFECTS OF DISPERSION AND ADSORPTION KINETICS ON COLUMN PERFORMANCE Introduction Empirical Characterization of Column Efficiency Modeling and Prediction of Column Efficiency GRADIENT ELUTION CHROMATOGRAPHY Introduction General Theory for Gradient Elution with Linear Isotherms LGE Relationships for Ion Exchange Chromatography LGE Relationships for RPC and HIC Separations with pH Gradients Modeling Gradient Elution with Non-linear Isotherms References DESIGN OF CHROMATOGRAPHIC PROCESSES Introduction Chromatographic Process Steps and Constraints Design for Capture Design for Chromatographic Resolution SMB Design References INDEX