The feasibility and mechanism of redox-active biochar for promoting anammox performance

Redox-active biochar has been regarded as an effective additive to promote heterotrophic denitrification, yet little is known about the feasibility of adding biochar for promoting anammox performance. In this study, we investigated the effects of different biochar doses (3-14 g/L; 1.5-7.1 g/g VSS) on anammox performance. Results showed that, in a short term (40 days), biochar could enhance anammox nitrogen removal rate (NRR) and nitrogen removal efficiency (NRE) by 0-18.0% and 0.2%-11.6%, respectively; this enhancement effect increased at 3-10 g biochar/L assays and reached a plateau at 10-14 g biochar/L assays. The optimal biochar dosage was identified to be 10 g/L (5.1 g/g VSS), with the NRR and NRE being 5.6%-18.0% and 4.0%-11.6% higher than those of the control, respectively. The highest specific anammox activity was simultaneously obtained at 10 g biochar/L assay, being 51% higher than that of the control. It revealed that biochar promoted the secretion of extracellular polymeric substances (increased by 30%-40% compared with that of the control) and increased the ratio of extracellular proteins to polysaccharides as well, directly enhancing the extracellular electron transfer capacity (ETC) of anammox biomass. The increased ETC of anammox biomass would further accelerate the metabolic activities of anammox bacteria, and promote the relative abundance of anammox bacteria, i.e., Ca. Brocadia was enriched by 5.8-12.6 folds than that of the control. These results demonstrate that biochar is feasible to enhance anammox activity and nitrogen removal performance, facilitating to a fast startup and enhanced nitrogen removal of anammox system.