染色质
生物
表观遗传学
质量细胞仪
组蛋白
组蛋白修饰酶
二价染色质
染色质重塑
细胞生物学
DNA甲基化
表观遗传学
遗传学
计算生物学
DNA
表型
基因表达
基因
作者
Peggie Cheung,Francesco Vallania,Hayley Warsinske,Michèle Donato,Steven Schaffert,Sarah Chang,Mai Dvorak,Cornelia L. Dekker,Mark M. Davis,Paul J. Utz,Purvesh Khatri,Alex Kuo
出处
期刊:Cell
[Elsevier]
日期:2018-05-01
卷期号:173 (6): 1385-1397.e14
被引量:240
标识
DOI:10.1016/j.cell.2018.03.079
摘要
Post-translational modifications of histone proteins and exchanges of histone variants of chromatin are central to the regulation of nearly all DNA-templated biological processes. However, the degree and variability of chromatin modifications in specific human immune cells remain largely unknown. Here, we employ a highly multiplexed mass cytometry analysis to profile the global levels of a broad array of chromatin modifications in primary human immune cells at the single-cell level. Our data reveal markedly different cell-type- and hematopoietic-lineage-specific chromatin modification patterns. Differential analysis between younger and older adults shows that aging is associated with increased heterogeneity between individuals and elevated cell-to-cell variability in chromatin modifications. Analysis of a twin cohort unveils heritability of chromatin modifications and demonstrates that aging-related chromatin alterations are predominantly driven by non-heritable influences. Together, we present a powerful platform for chromatin and immunology research. Our discoveries highlight the profound impacts of aging on chromatin modifications.
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