纳米团簇
荧光
X射线光电子能谱
材料科学
高分辨率透射电子显微镜
光化学
荧光光谱法
光谱学
谷胱甘肽
检出限
适体
化学
透射电子显微镜
纳米技术
化学工程
有机化学
工程类
物理
生物
量子力学
酶
遗传学
色谱法
作者
Shenghao Xu,Teng Gao,Xiuying Feng,Yaning Mao,Pingping Li,Xiaojiang Yu,Xiang Liu
摘要
In this work, we develop a facile one-step strategy for rapidly preparing dual ligand co-functionalized fluorescent gold nanoclusters (Au NCs) and establish a "turn on" approach for the rapid and selective sensing of glutathione (GSH) in aqueous solution and living cells. The as-prepared Au NCs exhibited orange red fluorescence (λem = 608 nm), a long lifetime (5.62 μs), a large stokes shift (>300 nm), and considerable stability and were systematically characterized by using fluorescence spectroscopy, high-resolution transmission electron microscopy (HRTEM), dynamic light scattering (DLS), X-ray photoelectron spectroscopy (XPS), fluorescence lifetime, infrared (IR) spectroscopy and ultraviolet absorption spectroscopy. Based on the fluorescence recovery induced by competitive coordination with Cu2+ between Au NCs and GSH, the present "turn on" approach offers a high sensitivity and excellent selectivity toward GSH detection with a detection limit of 9.7 nM. More importantly, this "turn on" approach could also be successfully applied for visualizing and monitoring the changes in the intracellular GSH level in Hep G2 cells, providing available potential for diagnostic applications.
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