自噬
细胞生物学
聚腺苷酸
核糖核酸
信使核糖核酸
RNA结合蛋白
生物
ATG8型
ATG5型
袋3
化学
基因
生物化学
细胞凋亡
作者
Han-Jeong Hwang,Hong-Seok Ha,Ban Seok Lee,Bong Heon Kim,Hyun Kyu Song,Yoon Ki Kim
标识
DOI:10.1038/s41467-022-29139-1
摘要
Abstract LC3/ATG8 has long been appreciated to play a central role in autophagy, by which a variety of cytoplasmic materials are delivered to lysosomes and eventually degraded. However, information on the molecular functions of LC3 in RNA biology is very limited. Here, we show that LC3B is an RNA-binding protein that directly binds to mRNAs with a preference for a consensus AAUAAA motif corresponding to a polyadenylation sequence. Autophagic activation promotes an association between LC3B and target mRNAs and triggers rapid degradation of target mRNAs in a CCR4-NOT–dependent manner before autolysosome formation. Furthermore, our transcriptome-wide analysis reveals that PRMT1 mRNA, which encodes a negative regulator of autophagy, is one of the major substrates. Rapid degradation of PRMT1 mRNA by LC3B facilitates autophagy. Collectively, we demonstrate that LC3B acts as an RNA-binding protein and an mRNA decay factor necessary for efficient autophagy.
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