适体
化学
检出限
费斯特共振能量转移
核酸外切酶 III
荧光
胶体金
核酸外切酶
选择性
互补DNA
分析化学(期刊)
DNA
纳米颗粒
色谱法
纳米技术
分子生物学
材料科学
生物化学
DNA聚合酶
基因
物理
催化作用
生物
大肠杆菌
量子力学
作者
Ruike Liu,Baoshan He,Huali Jin,Zhiguang Suo
标识
DOI:10.1016/j.aca.2021.339329
摘要
A fluorescent aptasensor was constructed to detect lead ion (Pb2+). Complementary DNA with sulfhydryl group (SH-cDNA) were loaded on gold nanoflowers materials (Au NFs) through Au-S bonds. Then, based on complementary base pairing between FAM modified Pb2+ aptamer (FAM-Apt) and Au NFs/SH-cDNA system, an Au NFs/SH-cDNA/FAM-Apt aptasensor was constructed. The fluorescence of FAM was quenched by Au NFs due to the fluorescence resonance energy transfer (FRET). Upon addition of Pb2+ and RecJf exonuclease (RecJf Exo) for 1.5 h, the Apt changed to a G-quadruplex structure due to the high affinity between Pb2+ and its aptamer, which lead to a large recovery in the fluorescence intensity. Under the optimized conditions, the presented aptasensor revealed high selectivity toward Pb2+ in the concentration range of 0.5 nM-1 μM, with a limit of detection (LOD) of 0.285 nM. Besides, the designed aptasensor was successfully used to recognize Pb2+ with excellent selectivity, reproducibility and stability in tap water and tea, providing a potential platform for Pb2+ detection in actual samples.
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