检出限
纳米团簇
辣根过氧化物酶
化学
荧光
双金属片
猝灭(荧光)
胶体金
免疫分析
纳米颗粒
色谱法
核化学
分析化学(期刊)
光化学
催化作用
材料科学
纳米技术
酶
生物化学
抗体
有机化学
物理
量子力学
免疫学
生物
作者
Zhenjiang Liu,Xiaoyan Wang,Xuexiang Ren,Weibin Li,Qaiser Javed,Xinwei Wang,Yuqian Huang,Yanguo Guo,Huawei Zeng
出处
期刊:Food Chemistry
[Elsevier]
日期:2021-09-01
卷期号:355: 129633-129633
被引量:44
标识
DOI:10.1016/j.foodchem.2021.129633
摘要
In the presented study, a horseradish peroxidase (HRP)-mediated ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) for zearalenone (ZEN) was reported based on fluorescence quenching of gold-silver bimetallic nanoclusters (Au-Ag NCs). HRP-antibody was used as a bridge in this immunoassay, linking the ratiometric fluorescence signal to the ZEN concentration. HRP catalyzed the oxidization of o-phenylenediamine in the presence of H2O2, leading to the formation of 2,3-diaminophenazine, which not only delivered a new peak at 580 nm but also quenched Au-Ag NCs fluorescence at 690 nm. Under optimal conditions, the detection limit for the proposed ELISA was 0.017 ng/mL, which was approximately 6.6-fold lower than conventional ELISA. Moreover, analytical performances were evaluated fully including specificity, accuracy, precision, and practicability, and showed that this method provides a potential platform for sensitive and reliable detection of ZEN.
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