Estimation of molecular radius, free mobility, and valence using polyacrylamide gel electrophoresis

Abstract Relative mobility values for macromolecules in polyacrylamide gel electrophoresis at various gel concentrations are used to compute the retardation coefficient, KR, molecular radius, R , free mobility, M0, and valence, V. Automated data processing and formal statistical analysis are applied. Results obtained with 29 proteins in 9 electrophoretic systems illustrate the utility of this approach, and also provide estimates of radius and total length of the acrylamide polymer.