FOXP3型
固有层
白细胞介素2受体
调节性T细胞
免疫系统
T细胞
炎症
化学
生物
免疫学
分子生物学
细胞生物学
上皮
遗传学
作者
Masayuki Saruta,Qi Yu,Phillip Fleshner,Pierre‐Yves Mantel,Carsten B. Schmidt‐Weber,Alison H. Banham,Konstantinos A. Papadakis
标识
DOI:10.1016/j.clim.2007.08.003
摘要
FOXP3+CD4+ regulatory T cells (TR) have emerged as important regulators of immune responses. The aim of our study was to assess the frequency and functional characteristics of FOXP3+CD4+ TR in Crohn’s disease (CD). We report that FOXP3+CD4+ TR cells are expanded in mucosal lymphoid tissues (lamina propria and MLN) but are decreased in the PB in active CD. Patients treated with thiopurines, but not steroids or anti-TNF-α inhibitors, have a lower frequency of PB FOXP3+CD4+ TR (7.8 ± 2.4% vs. 9.9 ± 1.8%, p = 0.01). FOXP3+ cells were localized in the lamina propria (LP), muscularis mucosa and serosa and accumulated in granulomas, whereas in MLN they localize in the T cell rich areas. MLN CD4+CD25+ T cells from both CD and normal intestine efficiently suppress the proliferation of effector CD4+CD25− T cells. T cell activation of MLN in vitro with anti-CD3 plus anti-CD28 Abs enhances the expression of FOXP3, both at the protein and transcriptional level, which is further enhanced by the addition of TGF-β. In summary, there is an expansion of FOXP3+CD4+ TR cells in mucosal lymphoid tissues in CD; they accumulate in areas of active inflammation, including granulomas and retain potent regulatory activity ex vivo.
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