清脆的
生物制氢
基因组编辑
暗发酵
生物过程
生化工程
CRISPR干扰
生物技术
梭菌
生物
计算生物学
计算机科学
制氢
细菌
基因
遗传学
工程类
生物化学
催化作用
古生物学
作者
Cut Ulfah Nihayati Husaini,Rozieffa Roslan,Ahmad Bazli Ramzi,Abdullah Amru Indera Luthfi,Jian Ping Tan,Swee Su Lim,Gong Tao Ding,Jamaliah Md Jahim,Peer Mohamed Abdul
标识
DOI:10.1016/j.ijhydene.2023.03.162
摘要
Generating hydrogen gas (H2) using the dark fermentation method has attracted much attention due to its lower energy requirement and environmental friendliness. However, producing a high yield of bio-H2 is as challenging as ever due to low energy conversion by microorganisms. In this respect, the advancement of genome editing tools including the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas technology could overcome the established maximum ceiling of product yield. To date, CRISPR-Cas systems, particularly those based on Type II CRISPR-Cas9 and Type V CRISPR-Cas12, are widely used in manipulating novel bacteria to improve the yield of specific biofuel. However, studies using the CRISPR-Cas technology for improving bio-H2 production remain scarce. Understanding the metabolic pathways of Clostridium spp. is essential for using the CRISPR-Cas technology Thus, this review highlighted the state-of-the-art in CRISPR-Cas systems for bacterial genome editing while paying attention to bioprocess optimization strategies for modulating the biohydrogen production.
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