枯草芽孢杆菌
生物过程
甘油
基质(水族馆)
大肠杆菌
生物反应器
肺炎克雷伯菌
效价
生物柴油生产
食品科学
化学
生物柴油
微生物学
生物技术
生物
细菌
生物化学
催化作用
病毒学
古生物学
病毒
有机化学
基因
遗传学
生态学
作者
Abhroop Garg,Carsten Jers,Hee Jin Hwang,Aida Kalantari,Ildze Ventina,Ivan Mijakovic
标识
DOI:10.3389/fbioe.2023.1101232
摘要
3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae . We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP.
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