加巴能
神经科学
中缝背核
抑制性突触后电位
光遗传学
心理学
生物
血清素
5-羟色胺能
遗传学
受体
作者
Donald Ielpo,Serafina Manila Guzzo,Giovanni F. Porcheddu,Maria Teresa Viscomi,Clarissa Catale,Ingrid Reverte,Simona Cabib,Carlo Cifani,Gabriella Antonucci,Rossella Ventura,Luisa Lo Iacono,Carla Marchetti,Diego Andolina
标识
DOI:10.1073/pnas.2301730120
摘要
The brain employs distinct circuitries to encode positive and negative valence stimuli, and dysfunctions of these neuronal circuits have a key role in the etiopathogenesis of many psychiatric disorders. The Dorsal Raphè Nucleus (DRN) is involved in various behaviors and drives the emotional response to rewarding and aversive experiences. Whether specific subpopulations of neurons within the DRN encode these behaviors with different valence is still unknown. Notably, microRNA expression in the mammalian brain is characterized by tissue and neuronal specificity, suggesting that it might play a role in cell and circuit functionality. However, this specificity has not been fully exploited. Here, we demonstrate that microRNA-34a (miR-34a) is selectively expressed in a subpopulation of GABAergic neurons of the ventrolateral DRN. Moreover, we report that acute exposure to both aversive (restraint stress) and rewarding (chocolate) stimuli reduces GABA release in the DRN, an effect prevented by the inactivation of DRN miR-34a or its genetic deletion in GABAergic neurons in aversive but not rewarding conditions. Finally, miR-34a inhibition selectively reduced passive coping with severe stressors. These data support a role of miR-34a in regulating GABAergic neurotransmitter activity and behavior in a context-dependent manner and suggest that microRNAs could represent a functional signature of specific neuronal subpopulations with valence-specific activity in the brain.
科研通智能强力驱动
Strongly Powered by AbleSci AI