火山盐蕨
鉴定(生物学)
生物
盐古菌
遗传学
计算生物学
古细菌
细菌
植物
作者
Marius Ortjohann,Peter Schönheit
出处
期刊:Fems Microbiology Letters
[Oxford University Press]
日期:2024-04-08
标识
DOI:10.1093/femsle/fnae026
摘要
Ketohexokinase (KHK) catalyzes the ATP-dependent phosphorylation of fructose, forming fructose-1-phosphate and ADP. The enzyme is well studied in Eukarya, in particular in human and other vertebrates, but homologs have not been identified in Bacteria and Archaea. Here we report the identification of a novel type of KHK from the haloarchaeon Haloferax volcanii (HvKHK). The encoding gene khk was identified as HVO_1812. The gene was expressed as 90 kDa homodimeric protein, catalyzing the phosphorylation of fructose with a Vmax value of 59 U/mg and apparent KM values for ATP and fructose of 0.47 mM and 1.29 mM, respectively. Homologs of HvKHK were only identified in few haloarchaea and halophilic Bacteria. The protein showed low sequence identity to characterized KHKs from eukaryotes and phylogenetic analyses indicate that haloarchaeal KHKs are largely separated from eukaryal KHKs. This is the first report of the identification of KHKs in prokaryotes that form a novel cluster of sugar kinases within the ribokinase/pfkB superfamily.
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