Lipoic acid-kaempferol hydrogel loaded with Typhaneoside for sutureless wound closure, hemostasis, and healing of diabetic infected wounds

Conventional hemostatic materials face challenges in adequately addressing the requirements of patients suffering from non-compressible hemorrhage. Furthermore, diabetic infected wounds frequently present a persistent and slow-healing condition. Currently available treatment strategies are unable to fully address non-compressible hemorrhage and promote the healing of diabetic infected wounds. In this study, we prepared a hydrogel named LTK, which is cross-linked by reversible hydrogen bonds and coordination bonds, using lipoic acid(LA)and kaempferol (Ka) as the main raw materials, and loaded it with Typhaneoside(TYP). The LTK-TYP hydrogel can fully diffuse into the irregularities of substrate surfaces and robustly adhere to various substrates. Molecular dynamics simulations further unveiled the atomic details of the formation of the LTK-TYP hydrogel through multi-modal physical interactions. In vitro and in vivo studies demonstrated that the LTK-TYP hydrogel exhibits excellent hemostatic ability, biocompatibility, and wound closure capacity. Moreover, it promotes the healing of diabetic methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds through antibacterial, anti-inflammatory, and angiogenic functions. This study showcases the immense potential of this multifunctional hydrogel in wound closure, hemostasis, and the healing of diabetic infected wounds. This study introduces a multifunctional hydrogel named LTK-TYP, composed of lipoic acid (LA) and kaempferol (Ka) cross-linked by reversible hydrogen and coordination bonds, and loaded with Typhaneoside (TYP). It exhibits strong adhesion to irregular surfaces. Molecular dynamics simulations reveal its formation through multi-modal interactions. In vitro and in vivo experiments demonstrate its superior hemostatic ability, biocompatibility, and wound closure capacity. Additionally, LTK-TYP promotes healing of diabetic MRSA-infected wounds through antibacterial, anti-inflammatory, and angiogenic functions, showing great potential for wound closure, hemostasis, and diabetic wound healing. • Through reversible hydrogen bonds and coordination bonds, we have successfully prepared a supramolecular interaction-driven bioadhesive hydrogel, LTK-TYP. • It can form robust adhesion with a variety of substrates. • Superior hemostatic performance, reducing blood loss and shortening bleeding time. • It promotes the healing of diabetic MRSA-infected wounds through antibacterial, anti-inflammatory, antioxidant, and angiogenic functions.