Addressable DNA Information Processing System with a Fluorescent Readout for Rewritable Memory

Comprehensive Summary DNA‐based nanostructure allows the construction of molecular devices useful in biological computing and information processing. Herein, an addressable and editable DNA information processing system established on a fluorescence intensity signal detection platform to save and encrypt information is proposed. The system operates by encoding information into distinct and changeable units of the trigger strands decoding by fluorescence intensity signal detection. Through toehold‐mediated strand displacement reactions, the trigger strand can be precisely added to and removed from the memorizer and reporter to implement the function of editing, encrypting, and decrypting. Our strategy is simple to implement, requiring only two mixing steps at room temperature for each operation and fluorescence intensity signal detection to read the data. And the system can realize accurate retrieval of specific individual information, eliminating all unnecessary redundant readouts. Because of its point‐to‐point accurate readout and programmability, the system is expected to become a powerful tool for the future development of information storage and sensing of biological molecules.