突变体
生物
镉
野生型
过氧化氢酶
互补
细胞生物学
转录因子
基因
植物
分子生物学
遗传学
生物化学
氧化应激
化学
有机化学
作者
Shubao Hu,Yao Yu,Qiuhong Chen,Guangmao Mu,Zhenguo Shen,Luqing Zheng
标识
DOI:10.1016/j.plantsci.2017.08.002
摘要
Cadmium (Cd) is one of the most toxic heavy metal elements in nature, and it causes serious damage to plant cells. Here, we report that a transcription factor OsMYB45 is involved in Cd stress response in rice. OsMYB45 is highly expressed in rice leaves, husks, stamens, pistils, and lateral roots, and its expression is induced by Cd stress. OsMYB45 fused to green fluorescent protein localized to the cell nucleus in onion epidermal cells. Mutation of OsMYB45 resulted in hypersensitivity to Cd treatment, and the concentration of H2O2 in the leaves of mutant nearly doubled, while catalase (CAT) activity was halved compared with the wild-type. Moreover, gene expression analysis indicated that OsCATA and OsCATC expression is significantly lower in the mutant than in the wild-type. In addition, overexpression of OsMYB45 in the mutant complemented the mutant phenotype. Taken together, OsMYB45 plays an important role in tolerance to Cd stress in rice.
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