HPLC-DAD-ESI-MS Fingerprinting and Antioxidant Potential of Phenolic Constituents Extracted from Martynia annua

Martynia annua Linn.(Family: Martyniceae), is a medicinally important plant being rich in phenolics.The current study was undertaken to investigate the phenolic acids and flavonoid glycosides using HPLC-DAD-ESI-MS fingerprinting and explore their total phenolic content (TPC), total flavonoid content (TFC) and antioxidant potential.Various parts of M. annua were extracted by ultrasound assisted solvent extraction and HPLC method was developed.Based on HPLC data, UV absorption and MS profiling, 23 compounds were putatively identified.Apigenin, luteolin, hispidulin and quercetin glycosides were the major flavonoid glycosides.Leaves and flower showed higher TPC (226.84 and 49.31 µg/mL respectively) and TFC (152.02 and 122.22 µg/mL respectively) and have promising antioxidant activity.So, it is demonstrated that, extracts from M. annua have high phenolic and flavonoid contents and antioxidant capacities.