生物
清脆的
谱系(遗传)
电池类型
斑马鱼
计算生物学
转录组
达尼奥
模式生物
基因组编辑
基因
遗传学
细胞
基因组
基因表达
作者
Bastiaan Spanjaard,Bo Hu,Nina Mitic,Pedro Olivares-Chauvet,Sharan Janjuha,Nikolay Ninov,Jan Philipp Junker
摘要
A key goal of developmental biology is to understand how a single cell is transformed into a full-grown organism comprising many different cell types. Single-cell RNA-sequencing (scRNA-seq) is commonly used to identify cell types in a tissue or organ. However, organizing the resulting taxonomy of cell types into lineage trees to understand the developmental origin of cells remains challenging. Here we present LINNAEUS (lineage tracing by nuclease-activated editing of ubiquitous sequences)-a strategy for simultaneous lineage tracing and transcriptome profiling in thousands of single cells. By combining scRNA-seq with computational analysis of lineage barcodes, generated by genome editing of transgenic reporter genes, we reconstruct developmental lineage trees in zebrafish larvae, and in heart, liver, pancreas, and telencephalon of adult fish. LINNAEUS provides a systematic approach for tracing the origin of novel cell types, or known cell types under different conditions.
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