Engineering a vitamin B12 high-throughput screening system by riboswitch sensor in Sinorhizobium meliloti

To our knowledge, it is the first time that a riboswitch element was used in S. meliloti. The flow cytometry high-throughput screening system was successfully developed and a high-yield VB₁₂ producing strain was obtained. The identified and analyzed gene mutations gave useful information for developing high-yield strains by metabolic engineering. Overall, this work provides a useful high-throughput screening method for developing high VB₁₂-yield strains.