The overexpression of tribbles homolog 3 promotes the secretion of collagen I in mouse fibroblast cell

Objective The aim of this study is to investigate the effect on secretion of collagen with overexpression of tribbles homolog 3(TRB3) in mouse fibroblast 3T3-NIH.Methods Using adenoviral vectors to overexpress TRB3, and the expression of TRB3 was detected by PCR and Western blot. The expression of collagen was detected by Sirius red staining kit and Western blot. Results The expression of TRB3 was significantly increased after infecting adenoviral vector. After overexpress TRB3 specifically, the expression and secretion of type I collagen were up-regulated in the mouse fibroblast cells, but the expression of type III collagen was decreased. Conclusion The overexpression of TRB3 promotes the secretion and expression of type I collagen in mouse fibroblasts cells.