The components and contents of organic acids in pineapple mature fruits from 56 germplasms were determined by high performance liquid chromatography(HPLC).The results showed that the major components of organic acids in pineapple fruits were citric acid,malic acid and quinic acid.Citric acid was ranged from 1.80 mg/g FW to 8.09 mg/g FW,with a mean of 4.82 mg/g FW,and citric acid content was the highest value,which accounted for 64.73% of the value of organic acids,followed by quinic acid and malic acid.The variation range of quinic acid was relatively stable among different germplasms,while the value of citric acid and malic acid varied greatly,showing as 33.86% and 37.92%,respectively.All the germplasms could be classified into citric acid dominant based on their organic acid composition.