Expression of Soluble Proteins Eimeria.acervulina 3-1E Gene in E.coli under Low Temperature

The sequences of Eimeria.acervulina 3-1E gene were amplified by RT-PCR.The processed 3-1E gene was connected to PET28a,an expression vector by EcoRⅠand NotⅠdouble digestion and transformed into Escherichia coli BL21(DE3).The recombinant expression vectors was identified by double digestion,and sequenced.The highest soluble expression level of recombinant protein was obtained after 50h at 16℃ and the concentration of IPTG was 0.4mmol/L.