Changes of IL-8 and IL-10 in lung tissues with hyperoxia-induced injury

Objective To study the changes of interleukin 8(IL-8)and interleukin 10(IL-10)in hyperoxia-induced lung injury and significances.Methods The model of rat with hyperoxia-induced lung injury was established by being inhaled with high concentration of oxygen(95%).The lung tissues were harvested 6hand 1,3,6,10,14dafter oxygen treatment,the histopathological changes of lung tissues were observed by HE staining and Double-antigen sandwich ELISA was used to detect the IL-8and IL-10levels in the lung tissue homogenate.Results The infiltrative inflammatory cells appeared in alveolus first,and then alveolus was enlarged,then alveolar septum became thinner and eventually the number of alveolus was decreased as the time of exposure to oxygen prolonged. The concentrations of IL-8in lung tissue homogenate in high oxygen group were significantly higher than those in control group,the level reached the peak at day 3after high oxygen exposure and the statistical difference lasted until the 14th day.The concentrations of IL-10in lung tissue homogenate were significantly higher after high oxygen exposure 6hand also had statistical difference on day 1,but restored normal level at day 3.Conclusion The concentration of inflammatory factor IL-8is increased fast and lasts for long time while anti-inflammatory factor IL-10is increased for short time in hyperoxia-induced lung injury.