Chinese medicine Sheng Jing Decoction alleviates oligoasthenospermia by modulating PI3K/Akt and MAPK signaling pathways

• Sheng Jing Decoction (SJD) ameliorates triptolide-induced oligoasthenospermia, restoring sperm concentration, motility, and spermatogenesis in mice. • The therapeutic effect of SJD is mediated through the concurrent modulation of the PI3K/Akt and MAPK signaling pathways. • SJD exerts multi-protective effects against testicular toxicity by mitigating apoptosis, oxidative stress, and inflammation. • This study provides a mechanistic foundation for the traditional use of SJD as a potential complementary therapy for male infertility.