基因敲除
钙粘蛋白
下调和上调
生物
长非编码RNA
癌症研究
细胞生长
转移
细胞
细胞凋亡
基因
癌症
遗传学
作者
Shusen Zheng,Huimou Chen,Yingxue Wang,Wenchao Gao,Zhiqiang Fu,Quanbo Zhou,Yanjun Jiang,Lin Q,Langping Tan,Huilin Ye,Xiaohui Zhao,Yuming Luo,Guolin Li,Liangtao Ye,Yimin Liu,Wenzhu Li,Zhihua Li,Rufu Chen
出处
期刊:Cancer Letters
[Elsevier]
日期:2016-02-01
卷期号:371 (2): 354-365
被引量:54
标识
DOI:10.1016/j.canlet.2015.12.010
摘要
Long non-coding RNAs (lncRNAs) are important regulators in pathological processes, yet their potential roles in pancreatic ductal adenocarcinoma (PDAC) are poorly understood. Here, we found that a novel lncRNA, LOC389641, was upregulated in PDAC tissues and cell lines. The expression of LOC389641 was significantly correlated with staging, lymph node metastasis and overall survival. Knockdown of LOC389641 impaired cell proliferation and invasion and induced cell apoptosis in vitro, whereas overexpression of LOC389641 had the opposite effect. The growth promoting effect of LOC389641 was also demonstrated in vivo. Further, a significant negative correlation was observed between E-cadherin levels and LOC389641 levels in vivo. Knockdown of LOC389641 upregulated E-cadherin expression, but knockdown of E-cadherin had a limited influence on LOC389641. Importantly, after E-cadherin was inhibited, the enhancement of LOC389641 on cell invasion was hindered. Moreover, the expression of LOC389641 was closely associated with its genomic neighboring gene TNFRSF10A. Lastly, knockdown experiments showed that TNFRSF10A might be a connection between LOC389641and E-cadherin. We conclude that LOC389641 promotes PDAC progression and increases cell invasion by regulating E-cadherin with the possible involvement of TNFRSF10A.
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