CTCF公司
粘蛋白
染色质
生物
细胞生物学
活体细胞成像
遗传学
计算生物学
细胞
DNA
基因
增强子
基因表达
作者
Michele Gabriele,Hugo B. Brandão,Simon Grosse-Holz,Asmita Jha,Gina M. Dailey,Claudia Cattoglio,Tsung-Han S. Hsieh,Leonid A. Mirny,Christoph Zechner,Anders J. Hansen
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2022-04-14
卷期号:376 (6592): 496-501
被引量:59
标识
DOI:10.1126/science.abn6583
摘要
Animal genomes are folded into loops and topologically associating domains (TADs) by CTCF and loop-extruding cohesins, but the live dynamics of loop formation and stability remain unknown. Here, we directly visualized chromatin looping at the Fbn2 TAD in mouse embryonic stem cells using super-resolution live-cell imaging and quantified looping dynamics by Bayesian inference. Unexpectedly, the Fbn2 loop was both rare and dynamic, with a looped fraction of approximately 3 to 6.5% and a median loop lifetime of approximately 10 to 30 minutes. Our results establish that the Fbn2 TAD is highly dynamic, and about 92% of the time, cohesin-extruded loops exist within the TAD without bridging both CTCF boundaries. This suggests that single CTCF boundaries, rather than the fully CTCF-CTCF looped state, may be the primary regulators of functional interactions.
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