Hesperetin modulation of oxidative stress and inflammatory mediators in LPS‐Activated BV‐2 Microglial Cells

氧化应激 橙皮素 活性氧 神经炎症 谷胱甘肽 化学 抗氧化剂 炎症 分子生物学 药理学 生物化学 生物 免疫学 类黄酮
作者
Jasmine Hunter Evans,Patricia Mendonca,Karam F. A. Soliman
出处
期刊:The FASEB Journal [Wiley]
卷期号:36 (S1) 被引量:1
标识
DOI:10.1096/fasebj.2022.36.s1.r2269
摘要

Neurodegeneration affects millions of Americans each year, occurring in later periods of life, where its onset is induced by neuroinflammation and oxidative stress. Flavonoids are known to protect against damages caused by reactive oxygen species (ROS) and reactive nitrogen species (RNS). This study evaluated the effect of hesperetin (HST), a major flavonoid found in oranges and mandarins, on LPS-activated BV-2 microglial cells. The obtained results of RT-PCR showed that HST down-regulated the mRNA expression of PD-L1 in more than 50% of the LPS-activated cells. In addition, HST induced the mRNA expression of Nrf2, which is involved in the transcription of several antioxidant genes. Moreover, the data from the oxidative stress PCR arrays showed that after 24 h, the concentration of 100 µM of HPT modulated numerous genes that regulate oxidative stress and inflammatory processes. HST down-regulated the mRNA expression of ERCC6, ACTB, SQSTM11, NOS2, and NCF1 and up-regulated the expression of HMOX1, which participate not only in excessive oxidative stress processes, by also in exacerbated inflammatory states. Both CAT and SOD enzymatic assays showed that treatment with HSP and LPS alone had no effect on CAT expression, but when cells were treated with the combination of HST and LPS, there was a reduction in these expressions enzymes. The quantitative glutathione assays demonstrated that only the combination treatment of HST and LPS reduced the expression of glutathione after a 24-h treatment period. In conclusion, these data show that hesperetin modulates several genes associated with oxidative stress and neuroinflammation and may potentially slow the progression of neurodegeneration.

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