内吞作用
类有机物
毒性
肠细胞
细胞生物学
胃肠道
网格蛋白
微塑料
细胞凋亡
化学
肠粘膜
生物
小肠
细胞
生物化学
医学
内科学
有机化学
环境化学
作者
Zongkun Hou,Run Meng,Jigang Bao,Tangmin Lai,Rui Qu,Shilei Hao,Dechang Jia,Bochu Wang
标识
DOI:10.1016/j.scitotenv.2022.155811
摘要
Plastic particles, especially nanoplastics, represent an emerging concern of threat to human health, oral uptake is an important pathway for the plastic particles ingestion by human. While their fate and adverse effects in animal gastrointestinal tract are increasingly investigated, knowledge about their uptake and toxicity in human intestine is still limited. Here, by exposing human intestinal organoids to polystyrene nanoplastics (PS-NPs, ~50 nm in size) with concentrations of 10 and 100 μg/mL, we present evidence of their distinct accumulation in various type cells in intestinal organoids, then causing the cell apoptosis and inflammatory response. Our results further revealed that the effective inhibition of PS-NPs accumulation in secretive cells through co-exposure to a clathrin-mediated endocytosis inhibitor (chlorpromazine), and proved the essential role of active endocytosis in the PS-NPs uptaking into enterocyte cells. Our work not only elucidated the potential uptake and toxicity of PS-NPs in human intestinal cells and the underlying mechanism, but also provide a potential therapeutic approach to relieve the toxicity of PS-NPs to human through the endocytosis inhibition.
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