肾毒性
庆大霉素
细胞毒性
流式细胞术
基因表达
细胞凋亡
体外
化学
药理学
毒性
基因
活力测定
生物
分子生物学
生物化学
抗生素
有机化学
作者
Maria Augusta Amaral Campos,Leonardo Augusto de Almeida,Marina Felipe Grossi,Carlos Alberto Tagliati
摘要
Abstract Acute renal failure is one of the most frequent effects observed after taking medicine. Such situations have been tardily discovered, given that existing methods for assessing toxicity are not predictive. In this light, the present work evaluated the effects of gentamicin, a form of nephrotoxic drug, on HK‐2 and HEK‐293 cells. By using MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) and flow cytometry, both cells demonstrated that cytotoxicity occurs in a dose‐dependent manner through the processes of apoptosis and cell necrosis. Gene expression analysis showed a relative increase of expression for genes related to cell processes and classic biomarkers, such as TP53, CASP3, CASP8, CASP9, ICAM‐1, EXOC3, KIM‐1, and CST3. A decrease in expression for genes BCL2L1 and EGF was observed. This study, therefore, indicates that, when the methods are used together, gene expression analysis is able to evaluate the nephrotoxic potential of a substance.
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