氮化碳
辅因子
材料科学
石墨氮化碳
光催化
碳纤维
醇脱氢酶
NAD+激酶
氮化物
生物催化
光化学
化学工程
化学
催化作用
酶
有机化学
反应机理
复合材料
复合数
工程类
图层(电子)
作者
Eun Jin Son,Yang Woo Lee,Jong Wan Ko,Chan Beum Park
出处
期刊:ACS Sustainable Chemistry & Engineering
[American Chemical Society]
日期:2018-12-27
卷期号:7 (2): 2545-2552
被引量:48
标识
DOI:10.1021/acssuschemeng.8b05487
摘要
In situ regeneration of nicotinamide cofactor (NADH) is imperative because it is required as a reducing power for driving many redox enzymatic cycles useful in industry. Here, we report that amorphous carbon nitride (ACN) is a promising and robust photocatalyst for solar-driven biotransformation via NADH regeneration. Under visible light (λ > 420 nm), NADH regeneration yields by ACN reached 62.3% within an hour, whereas partially crystalline polymeric carbon nitride (CCN) hardly reduced NAD+ to NADH. Subsequently, the regenerated cofactor was consumed by l-glutamate dehydrogenase, a NADH-dependent enzyme, achieving the conversion of α-ketoglutarate with a turnover frequency of 2640 h–1. ACN showed excellent catalytic activity and long-term stability for light-driven biocatalysis; NADH regeneration efficiency after eight cycles remained above 92% of the first cycle’s efficiency, and the enzymatic reaction proceeded for more than 12 h without significant loss of ACN’s photoactivity. The remarkable photocatalytic activity of ACN originated from its unique microstructure that lacks hydrogen bonds that link polymeric melon units, leading to extended visible light absorption and less charge recombination. Our results suggest that ACN efficiently drives biocatalytic photosynthesis with exceptional catalytic sustainability.
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