效应器
生物
分泌物
质外体
细胞生物学
大豆疫霉
植物免疫
蛋白酶
微生物学
生物化学
病菌
细胞壁
酶
基因
突变体
拟南芥
作者
Baodian Guo,H. Wang,Bo Yang,Wei Jiang,Maofeng Jing,H. Li,Yeqiang Xia,Yongjin Xu,Qinli Hu,F. Wang,Feifei Yu,Y. Wang,W. Ye,Suomeng Dong,Weiman Xing
标识
DOI:10.1016/j.molp.2019.01.017
摘要
Plants secrete defense molecules into the extracellular space (the apoplast) to combat attacking microbes. However, the mechanisms by which successful pathogens subvert plant apoplastic immunity remain poorly understood. In this study, we show that PsAvh240, a membrane-localized effector of the soybean pathogen Phytophthora sojae, promotes P. sojae infection in soybean hairy roots. We found that PsAvh240 interacts with the soybean-resistant aspartic protease GmAP1 in planta and suppresses the secretion of GmAP1 into the apoplast. By solving its crystal structure we revealed that PsAvh240 contain six α helices and two WY motifs. The first two α helices of PsAvh240 are responsible for its plasma membrane-localization and are required for PsAvh240’s interaction with GmAP1. The second WY motifs of two PsAvh240 molecules form a handshake arrangement resulting in a handshake-like dimer. This dimerization is required for the effector's repression of GmAP1 secretion. Taken together, these data reveal that PsAvh240 localizes at the plasma membrane to interfere with GmAP1 secretion, which represents an effective mechanism by which effector proteins suppress plant apoplastic immunity.
科研通智能强力驱动
Strongly Powered by AbleSci AI