免疫突触
表面改性
细胞生物学
DNA
细胞
蛋白质工程
膜蛋白
转导(生物物理学)
化学
膜
生物
免疫系统
生物物理学
T细胞
生物化学
免疫学
T细胞受体
物理化学
酶
作者
Mengyi Xiong,Gezhi Kong,Liu Q,Lu Liu,Yin Yao,Ying Liu,Hui Yuan,Xiaobing Zhang,Weihong Tan
出处
期刊:Nano Letters
[American Chemical Society]
日期:2022-12-28
卷期号:23 (1): 183-191
被引量:4
标识
DOI:10.1021/acs.nanolett.2c03928
摘要
Membrane protein engineering exhibits great potential for cell functionalization. Although genetic strategies are sophisticated for membrane protein engineering, there still exist some issues, including transgene insertional mutagenesis, laborious, complicated procedures, and low tunability. Herein, we report a DNA-templated anchoring of exogenous proteins on living cell membranes to realize programmable functionalization of living cells. Using DNA as a scaffold, the model cell membranes are readily modified with proteins, on which the density and ratio of proteins as well as their interactions can be precisely controlled through predictable DNA hybridization. Then, the natural killer (NK) cells were engineered to gain the ability to eliminate the immune checkpoint signaling at the NK-tumor synapse, which remarkably promoted NK cell activation in immunotherapy. Given the versatile functions of exogenous proteins and flexible designs of programmable DNA, this method has the potential to facilitate membrane-protein-based cell engineering and therapy.
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