间充质干细胞
沃顿果冻
肝细胞生长因子
脐带
男科
流式细胞术
血管内皮生长因子
CD90型
分泌物
活力测定
干细胞
人口
生物
化学
免疫学
细胞生物学
细胞
医学
川地34
内分泌学
生物化学
癌症研究
血管内皮生长因子受体
受体
环境卫生
作者
Arfianti Arfianti,Ulfah Ulfah,Leopold Sampetua Hutabarat,AIYADURAI S.G,Anisa Dwi Budiarti,Nabilla Sonia Sahara,Nicko Pisceski Kusika Saputra
标识
DOI:10.37796/2211-8039.1416
摘要
Background: Mesenchymal stem cell (MSC) has great potential as therapies due its ability to regenerate tissue damage and promote tissue homeostasis. Preconditioning of MSC in low oxygen concentration has been shown to affect the therapeutic potential of these cells. This study aimed to compare the characteristic and secretion of trophic factors of MSCs cultured under hypoxia and normoxia. Methods: MSCs were isolated from Wharton's jelly of human umbilical cord (UC) tissue by explant method and characterized by flow cytometry. Following 24 hours of CoCl2induced hypoxic culture, the viability and metabolic activity of MSC were analyzed by trypan blue exclusion test and methyl thiazolyl tetrazolium (MTT) assay, respectively. The secretion of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) was assessed in conditioned medium using enzyme-linked immunosorbent assay (ELISA) method. Results: Flow cytometry analysis showed >99% of the population of MSCs cells were positive for CD73 and CD90 and >62% were positive for CD105. While the cell viability of MSC was not affected by hypoxic cultured condition, the metabolic activity rate of these cells was decreased under hypoxic conditioning. In line with reduced metabolic activity, hypoxic human UC-derived MSC produced less HGF than normoxic counterpart. Compared to normoxic MSC, hypoxic preconditioned MSC secreted higher level of VEGF in the conditioned medium (p
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