Anti-gravity stem-seeking restoration algorithm for maize seed root image phenotype detection

• The proposed Anti-Gravity Stem-Seeking (AGSS) root image restoration algorithm can quickly and effectively repair root images. • AGSS root image restoration algorithm has good universality and does not need training data and high-performance hardware platforms. • Drought and waterlogging resistances of maizes can be effectively evaluated by the phenotypic parameters of repaired seed roots. Root phenotype detection is key to cultivating seeds with excellent traits, and requires a complete root image. However, soil occlusion, uneven lighting, and other factors cause broken points and segments in the root image. To solve this problem, an anti-gravity stem-seeking (AGSS) root image restoration algorithm is proposed in this paper to repair root images and extract root phenotype information for different resistant maize seeds. First, the obtained root image was processed using uniform illumination, grayscale, binarization, and morphological filtering to separate it from the background. Subsequently, a root skeleton map was generated using a thinning algorithm for pixel-level image processing, and the Taproot junction G was obtained. Subsequently, root pixel coordinates were obtained by traversing the root skeleton map. All root-segment endpoint coordinates were obtained using the endpoint judgment rule and stored in the endpoint list. The lateral and primary root endpoints were separated based on the lateral root judgment rule, and stored in the side root and primary root endpoint lists, respectively. Subsequently, the primary root endpoints were processed and fitted in the order short to long using arbitrary-two-endpoint spacing until all breakpoints of the primary root were found. The coordinates of the top endpoints of each principal root were obtained. Finally, the top endpoint was connected to point G based on the Bezier curve-fitting method to achieve complete root repair. The proposed AGSS root image restoration algorithm was applied to detect the root systems of maize with different resistances and wheat to evaluate its performance against the standard dataset. The results indicated a detection accuracy of greater than 90% for root taproot length and diameter. It was also found that maize drought resistance was positively correlated with root length and diameter, but negatively with the lateral root number. In contrast, the waterlogging and salt resistance traits of maize were positively correlated with the number of lateral roots. In conclusion, the proposed AGSS root image restoration algorithm can quickly and effectively repair root images, is suitable for different resistance evaluations of maize seeds, and is conducive to the detection of root phenotypes. Compared with deep learning methods, this algorithm displays advantages of fast repair, low hardware platform requirements, and less requirement of training images. The algorithm is highly suitable for deploying in small embedded systems, with broad application prospects.