生物
生发中心
端粒
长寿
否定选择
牛痘
记忆B细胞
脾脏
B细胞
边缘地带
表型
遗传学
免疫学
病毒学
抗体
基因
基因组
重组DNA
作者
Pascal Chappert,François Huetz,Marie-Alix Espinasse,Fabrice Chatonnet,Louise Pannetier,Lucie Da Silva,Clara Goetz,Jérôme Mégret,Aurélien Sokal,Étienne Crickx,Ivan Nemazanyy,Vincent Jung,Chiara Guerrera,Sébastien Storck,Matthieu Mahévas,Antonio Cosma,Patrick Revy,Thierry Fest,Claude‐Agnès Reynaud,Jean–Claude Weill
出处
期刊:Immunity
[Elsevier]
日期:2022-10-01
卷期号:55 (10): 1872-1890.e9
被引量:12
标识
DOI:10.1016/j.immuni.2022.08.019
摘要
Memory B cells (MBCs) can persist for a lifetime, but the mechanisms that allow their long-term survival remain poorly understood. Here, we isolated and analyzed human splenic smallpox/vaccinia protein B5-specific MBCs in individuals who were vaccinated more than 40 years ago. Only a handful of clones persisted over such an extended period, and they displayed limited intra-clonal diversity with signs of extensive affinity-based selection. These long-lived MBCs appeared enriched in a CD21hiCD20hi IgG+ splenic B cell subset displaying a marginal-zone-like NOTCH/MYC-driven signature, but they did not harbor a unique longevity-associated transcriptional or metabolic profile. Finally, the telomeres of B5-specific, long-lived MBCs were longer than those in patient-paired naive B cells in all the samples analyzed. Overall, these results imply that separate mechanisms such as early telomere elongation, affinity selection during the contraction phase, and access to a specific niche contribute to ensuring the functional longevity of MBCs.
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