Effect of integrin α2 on adhesion of neuroblastoma cells to collagen

AIM: To study the effect of integrin α2 on adhesion of SK-N-SH neuroblastoma cells to collagen. METHODS: Adhesion of the SK-N-SH cells to immobilized collagen was tested with various concentration of Mg~ 2+ , Ca~ 2+ and with 10 μg/L anti-α2 monoclonal antibody (mAb) 6F1. A_ 570 was detected as adhesion cell numbers. RESULTS: Mg~ 2+ -dependent adhesion of SK-N-SH cells to type I collagen was increased significantly, with peak adhesion at concentration of 1 mmol/L Mg~ 2+ . A_ 570 with or without Mg~ 2+ was 0.59±0.03 and 0.25±0.01 respectively (P0.01). No effect of Ca~ 2+ was found on SK-N-SH cell adhesion. 6F1 (10 mg/L) blocked the adhesion completely. CONCLUSION: Integrin α2 regulates neuroblastoma cells adhesion to collagen and suggest that it may play a role in tumor cell migration, invasion and metastasis.