The malondiadehyde content and cell membrane permeability of five pot-cultured tobacco cultivars and lines under drought stress were studied. The results showed that the malondiadehyde content increased at first, and then decreased with the increase of drought stress, while the cell membrane permeability kept increasing during drought stress; after rewatering the cell membrane permeability soon recovered while the malondiadehyde content needed a long time to restore. The cell membrane permeability responded to some extent of drought stress and the stabilization of malondiadehyde content might be meaningful to drought resistant of tobacco.