Identification of impurities in Moxifloxacin by chromatographic two-dimensional spectroscopy correlation

OBJECTIVE To establis a qualitative analysis method to identify related substances of Moxifloxacin by correlation of spectra in chromatography.METHODS Standard chromatographic and spectral data obtained by high-performance liquid chromatograpy with diode array detection(HPLC-DAD) were developed and used in identification of peaks in samples by calculating correlation coefficients.RESULTS Peaks in chromatography can be identified accurately and rapidly through chromatographic system such as column and mobile phase change.The limit amount for identification was 30 ng.CONCLUSION The method is accurate and reproducible as a new and effective way to identify multi-components in drugs when relative retention times are incapable due to changes of chromatographic system.