Objective: To investigate the relationship between the semen pH values and abnormal semen liquefaction in male sterility.Methods: 1 680 male patients who were treated in the urological Department of our hospital from January 2009 to January 2011 were analyzed retrospectively,of which 786 patients were sterile male patients and 894 were healthy male patients.The semen pH values and the semen liquefaction time were detected and analyzed.Results: The semen pH values and the semen liquefaction time were significantly different between the sterile patients and the healthy patients,there was a significant difference(P0.05).The group of prolonged semen liquefaction time was significantly higher than the group of normal semen liquefaction time in the decrease rate of semen pH values(P0.05).The group of decreased semen pH values was significantly higher than the group of normal semen pH values in the prolongation rate of semen liquefaction time(P0.05).Conclusion: The pH values of semen and the abnormal semen liquefaction are closely related.