Characterization of Cucumber Mosaic Virus Isolates from California

Thirty cucumber mosaic virus (CMV) isolates from California were characterized biologically (host reaction and thermosensitivity), serologically (protein A sandwich-enzyme-linked immunosorbent assay [PAS-ELISA]) and physically (viral capsid protein migration in polyacrylamide gel electrophoresis [PAGE], peptide mapping, and migration of viral double-stranded RNAs [dsRNAs] in PAGE). All methods supported the classification of CMV isolates into two main subgroups, CMV-I and CMV-II (.)