毕赤酵母
可选择标记
生物
转化(遗传学)
异源的
多克隆站点
GenBank公司
克隆(编程)
表达式向量
基因
遗传学
计算生物学
载体(分子生物学)
异源表达
登录号(图书馆学)
重组DNA
计算机科学
程序设计语言
作者
Joan Lin‐Cereghino,Matthew Hashimoto,Allison Moy,James Castelo,Claire C. Orazem,Peter Kuo,See Xiong,Vishal V. Gandhi,Christopher T. Hatae,Alex W Chan,Geoff P. Lin-Cereghino
出处
期刊:Yeast
[Wiley]
日期:2008-03-10
卷期号:25 (4): 293-299
被引量:54
摘要
Abstract The methylotrophic yeast, Pichia pastoris, is widely used as a host organism for the expression of heterologous proteins. Currently, the Zeocin and blasticidin resistance genes are the only dominant selectable markers that can be used for primary selection of transformants. In this report we describe new expression vectors that can be used to select directly for P. pastoris transformants using G418 resistance conferred by a modified Tn903kan r gene. Compared to other dominant markers, this system is more economical and offers a higher transformation efficiency, due to the small sizes of the cloning vectors, pKAN B and pKANα B (GenBank Accession Nos EU285585 and EU285586, respectively). Additionally, multicopy transformants can be generated using these new vectors. Copyright © 2008 John Wiley & Sons, Ltd.
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