海藻糖
冷冻干燥
结晶
无定形固体
化学
色谱法
化学工程
材料科学
结晶学
有机化学
工程类
作者
Prakash Sundaramurthi,Raj Suryanarayanan
摘要
Lyoprotectants are stabilizers used to prevent denaturation of proteins during freeze-drying and subsequent storage. In order to be effective, lyoprotectants must be retained amorphous. The physical state of the lyoprotectant is usually characterized by powder X-ray diffractometry of the dried cake. While trehalose is widely used as a lyoprotectant, we report its crystallization during freeze-drying and point out why it may not become evident from characterizing the final lyophile. When an aqueous trehalose solution was cooled to −40 °C, ice was the only crystalline phase observed. However, upon annealing at −18 °C, crystallization of trehalose dihydrate was evident. During drying, the dihydrate dehydrated to substantially amorphous anhydrate. Therefore, analyzing the final dried product will not reveal crystallization of the lyoprotectant during freeze-drying. In light of the observed phase separation of trehalose in frozen solutions, its ability to serve as a lyoprotectant warrants further investigation.
科研通智能强力驱动
Strongly Powered by AbleSci AI