Bioinformatics analysis in type 1 diabetes increases retention of low-density lipoprotein in the atherosclerosis-prone area of the murine aorta

We read with great interest the recent report by Hagensen et al. [ [1] Hagensen M.K. Mortensen M.B. Kjolby M. et al. Type 1 diabetes increases retention of low-density lipoprotein in the atherosclerosis-prone area of the murine aorta. Atherosclerosis. 2017; 263: 7-14 Google Scholar ] in Atherosclerosis. The results of the report are very helpful, however, from our perspective, the authors' methods in bioinformatics analysis are inappropriate. Type 1 diabetes increases retention of low-density lipoprotein in the atherosclerosis-prone area of the murine aortaAtherosclerosisVol. 263PreviewIndividuals with type 1 diabetes mellitus are at high risk of developing atherosclerotic cardiovascular disease, but the underlying mechanisms by which type 1 diabetes accelerates atherosclerosis remain unknown. Increased retention of low-density lipoprotein (LDL) in atherosclerosis-prone sites of the diabetic vascular wall has been suggested, but direct evidence is lacking. In the present study, we investigated whether retention of LDL is increased in atherosclerotic-prone areas using a murine model of type 1 diabetes. Full-Text PDF Reply to “Bioinformatics analysis in type 1 diabetes increases retention of low-density lipoprotein in the atherosclerosis-prone area of the murine aorta”AtherosclerosisVol. 263PreviewWe would like to thank Li et al. [1] for their interest in our work. We apologize if we have given the impression that we used t-tests to conclude on individual differentially regulated genes. This is not the case. Our initial analysis of the data for individual differentially regulated genes, corrected for multiple testing, did not reveal any genes with genome-wide significance. Instead, we report in our paper an over-representation analysis [2], which we used to test for enrichment of genes from four gene sets that we had previously found to be associated with LDL retention in areas of flow disturbance [3]. Full-Text PDF