化学
堆积
适体
费斯特共振能量转移
荧光
光致发光
范德瓦尔斯力
卟啉
纳米结构
吸附
检出限
光化学
共价键
纳米技术
生物传感器
分子
光电子学
色谱法
物理化学
有机化学
材料科学
物理
量子力学
生物
生物化学
遗传学
作者
Jing Cui,Lun Kan,Zhenzhen Li,Longyu Yang,Minghua Wang,Linghao He,Yafei Lou,Yulin Xue,Zhi Hong Zhang
出处
期刊:Talanta
[Elsevier]
日期:2021-06-01
卷期号:228: 122060-122060
被引量:31
标识
DOI:10.1016/j.talanta.2020.122060
摘要
A fluorescent aptasensor based on porphyrin-based covalent organic framework (p-COF) and carbon dots (CDs) was constructed for detecting vascular endothelial growth factor 165 (VEGF165) and for imaging of the breast cancer cell line Michigan cancer foundation-7 (MCF-7). CDs synthesized with strong photoluminescence at λ∼380 nm were used as donors to label the VEGF165-targeted aptamers (AptVEGF/CDs). Additionally, the p-COF nanostructure comprised rich functional groups of CN on the surface and π-stacking planar nanostructure, resulting in the CDs adsorption via weakly π−π stacking, hydrogen bond and the Van der Waals force. Thereby, the fluorescence resonance energy transfer (FRET) occurred due to the close distance between the p-COF network and CDs, leading to the quenching of the fluorescence feature of CDs and p-COF. In the presence of VEGF165, the G-quadruplex was formed via the specific binding between VEGF165 and aptamer. It impelled that the release of partial VEGF165-AptVEGF/CDs complex, affording the fluorescence recovery of the sensing system to some extent. Consequently, the proposed AptVEGF/CDs/p-COF fluorescence biosensor offered excellent analytical performances for the VEGF165 detection, displaying a detection limit of 20.9 fg mL−1 within a wide linear range of the VEGF165 concentration of 1.0 pg mL−1–100 ng mL−1. The developed fluorescence biosensor was also used to determine VEGF165-overexpressed in MCF-7 cancer cells. Thereby, the present work can greatly widen the application of COFs in the development of aptasensors and cancer diagnosis.
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