生物
转化(遗传学)
报告基因
基因表达
基因
计算生物学
格斯报告系统
细胞生物学
生物技术
遗传学
作者
Yubing He,Tao Zhang,Hui Sun,Huadong Zhan,Yunde Zhao
标识
DOI:10.1038/s41438-020-00390-1
摘要
Reporters have been widely used to visualize gene expression, protein localization, and other cellular activities, but the commonly used reporters require special equipment, expensive chemicals, or invasive treatments. Here, we construct a new reporter RUBY that converts tyrosine to vividly red betalain, which is clearly visible to naked eyes without the need of using special equipment or chemical treatments. We show that RUBY can be used to noninvasively monitor gene expression in plants. Furthermore, we show that RUBY is an effective selection marker for transformation events in both rice and Arabidopsis. The new reporter will be especially useful for monitoring cellular activities in large crop plants such as a fruit tree under field conditions and for observing transformation and gene expression in tissue culture under sterile conditions.
科研通智能强力驱动
Strongly Powered by AbleSci AI