过氧亚硝酸盐
光漂白
体内
化学
生物物理学
体外
荧光
生物分子
临床前影像学
细胞生物学
纳米技术
生物化学
超氧化物
材料科学
生物
酶
生物技术
物理
量子力学
作者
Guoyu Jiang,Chunbin Li,Qingfang Lai,Xiang Liu,Qingqing Chen,Pengfei Zhang,Jianguo Wang,Ben Zhong Tang
标识
DOI:10.1016/j.snb.2020.129223
摘要
Peroxynitrite (ONOO−), a potent oxidative and nitrating reagent that can damage a variety of molecules in cells is reported to be closely related to various diseases. To determine the physiological or pathophysiological concentrations of ONOO− and to deeply understand its pathophysiological processes strongly rely on the development of more powerful detection methods. Hereof, we fabricated the first ratiometric fluorescent probe by using aggregation-induced emission luminogens (AIEgens) with superior photostability for efficient and reliable visualization of ONOO− both in vitro and in vivo. Probe BTCV-PN, obtained via simple two-step process, displayed superior photostability, remarkable sensitivity and outstanding specificity toward ONOO− through a ratiometric response mode. Furthermore, BTCV-PN was successfully applied for imaging of exogenous and endogenous ONOO− in live cells. Notably, in vivo imaging of ONOO− in live mice was also accomplished. The excellent performances plus the superior parameters of BTCV-PN including reliable ratiometric response and superior stability against photobleaching would not only make BTCV-PN a powerful tool-box for facilely investigating the cellular concentrations of ONOO−, but also open up an updated way for straightforward visualization of the pathophysiological process of ONOO− in live cells and animals. Elsevier B.V. All rights reserved.
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