Comparative Study of the Transfection Efficiency of Macrophage by Different Methods

Objective: To transfect siRNA into murine macrophage by different methods, and comparison of different transfection methods. Method: The cells were transfected by different methods with lipofectamine,Roche transfection reagent,electroporation and lentiviral vectors,The analysis of transfection efficiency of different methods by flow cytometer,levels of target mRNA and cell viability were determined. Result: The efficiency of Lentivirus-mediated siRNA transfection is the highest,up to 34. 75 ± 5. 30%,and the RNAi effect is best; Followed by Roche transfection reagent and electroporation,but electroporation cell viability lowest; Lipofectamine difficult to transfected,only 12. 17 ± 1. 53%,but cell viability of Lipofectamine was greatest. Conclusion: Through the comparison of the four kinds of murine macrophage transfection methods,the advantages and disadvantages of four kinds of transfection methods was acquired,and provides the basis for murine macrophage transfection.