Microcirculatory Study on Three Kinds of Anastomoses for Digestive Tract Reconstruction

Objective To compare microcirculation of anastomotic stoma and wound healing condition among three kinds of intestinal anastomoses.Methods 18 hybrid dogs all underwent Albert single-layer anastomosis,Albert-Lembert double-layer anastomosis and stapler anastomosis at proximal end,midpiece and distal end of small intestine.6 dogs underwent same anastomosis at same location.Second abdominal operation was performed respectively at day 3,5,7 after anastomosis.Before operation and day 3,5,7,after operation,microcirculation and wound healing were observed,changes of pathological morphology were examined,levels of SOD and MDA in tissue homogenates of anastomotic stoma were measured.Results After anastomoses of single-layer and stapler,the volume of regional blood flow and the microcirculatory flow conditon,and the densities of area and amount of micrangium at anastomotic stoma were all better than those of double-layer group.In first two groups,the damage of peroxidation,the inflammatory response and inversion of anastomotic stoma were slightest.Operative time of single group and stapler group was significantly shorter than that of double group.Conclusion The micrangium reconstruction and healing of anastomotic stoma in single-layer and stapler of intestinal anastomoses were better than those of double-layer anatomosis.